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JWH 250 5-hydroxyindole metabolite is a synthetic cannabinoid used in scientific research and laboratory experiments. It is a derivative of the cannabinoid JWH-018, which is a potent agonist of the CB1 and CB2 receptors. It is expected to be a metabolite of JWH 250 that would be detectable both in serum and in urine. Where to Buy JWH-250 Online, Where to Order JWH-250 Online, JWH-250 for sale Online

Molecular Structure Analysis

The molecular formula of JWH 250 5-hydroxyindole metabolite is C22H25NO3. Its formal name is 1-(5-hydroxy-1-pentyl-1H-indol-3-yl)-2-(2-methoxyphenyl)-ethanone. The InChi Code is InChI=1S/C22H25NO3/c1-3-4-7-12-23-15-19 (18-14-17 (24)10-11-20 (18)23)21 (25)13-16-8-5-6-9-22 (16)26-2/h5-6,-811,14-15,24H,3-4,7,12-13H2,1-2H3

The antibodies can show cross-reactivity towards other structures than the targeted one, thus expanding the usefulness of the assays, and also enabling the detection of new SC.

JWH-250 for sale Online

The JWH-018 assay had moderate to high cross-reactivity with 17 SC or SC metabolites. Order JWH-250 Online The JWH-250 assay had very limited cross-reactivity, with JWH-250-5-OH-pentyl and JWH-250-5-carboxypentyl metabolites at 50% being the only ones with a cross-reactivity >1%, compared to the JWH-250-4-OH calibrator. JWH-250 for sale Compared to a confirmation method using LC-MS/MS, the specificities and sensitivities for both methods were more than 95%.

Using the same immunalysis homogeneous enzyme immunoassay (HEIA ) K2 Spice kit with JWH-018 N-pentanoic acid as calibrator, Barnes et al. (2014) and Kronstrand, Brinkhagen, Birath-Karlsson, Roman, & Josefsson (2014) presented sensitivity/specificity results of 92/98% and 87/82%, respectively. In total, 76 SC and metabolites were tested and of these 19 were classified as moderately to highly cross-reactive. Neither the HEIA nor ELISA assays showed cross-reactivity with the newer SC, such as UR-144 or XLR-11, or metabolites.

Arntson et al. (2013) reported two enzyme-linked immunosorbent assays (ELISA) that were designed to detect the 5-OH metabolite of JWH-018 and the 4-OH metabolite of JWH-250 in urine.

In all these methods, urine samples are either chemically or enzymatically hydrolyzed previous to analysis, as the SC are known to form glucuronidated metabolites in urine. The predominant sample preparation methods used were LLE and SPE, with the exception of Moran et al. (2011) who diluted the urine samples with methanol and Yanes and Lovett (2012) who used a salting out technique (SALLE). For GC-MS methods, derivatization might be necessary before analysis (Emerson, Durham, Gidden, & Lay, 2013).